patmedfarПациентоориентированная медицина и фармацияPatient-Oriented Medicine and Pharmacy2949-1924LLC Izdatelstvo OKI10.37489/2949-1924-0132CEOKTRpatmedfar-224Research ArticleНЕВРОЛОГИЯNEUROLOGYКлеточно-специфическая экспрессия рецептора к следовым аминам 1 (TAAR1) в нейронах различных отделов головного мозга: транскриптомный анализ с использованием общедоступных баз данныхCell-specific expression of trace amine receptor 1 (TAAR1) in neurons across brain regions: transcriptome analysis using publicly available databaseshttps://orcid.org/0000-0002-4542-6860ФилимоновД. А.FilimonovD. A.

Филимонов Дмитрий Алексеевич — д. м. н., зам. директора по научной работе

Донецк

Dmitry A. Filimonov — Dr. Sci. (Med.), Deputy Director for Research 

Donetsk 

neuro.dnmu@gmail.comhttps://orcid.org/0000-0002-1037-2567КотловскийМ. Ю.KotlovskiyM. Yu.

Котловский Михаил Юрьевич — д. м. н., главный научный сотрудник

Москва

Mikhail Yu. Kotlovsky — Dr. Sci. (Med.), Chief Researcher

Moscow

m.u.kotlovskiy@mail.ruhttps://orcid.org/0000-0002-4596-6517ПотаповМ. П.PotapovM. P.

Потапов Максим Петрович — д. м. н., зав. кафедрой медицинской кибернетики с курсом медицинской информатики 

Ярославль

Maxim P. Potapov — Dr. Sci. (Med.), Head of the Department of Medical Cybernetics with a Course in Medical Informatics

Yaroslavl

mxp@mail.ruhttps://orcid.org/0009-0004-3019-144XБелоцерковскаяМ. А.BelotserkovskayaM. A.

Белоцерковская Маргарита Андреевна — младший научный сотрудник

Донецк

Margarita A. Belotserkovskaya — Junior Researcher 

Donetsk 

margarita-amb@mail.ruhttps://orcid.org/0000-0001-9755-1869ПопандопулоА. Г.PopandopuloA. G.

Попандопуло Андрей Геннадиевич — д. м. н., профессор, зав. лабораторией клеточного и тканевого культивирования

Донецк

Andrey G. Popandopulo — Dr. Sci. (Med.), Professor, Head of the Department Laboratory of Cell and Tissue Cultivation

Donetsk 

PAG.lctc@mail.ruhttps://orcid.org/0009-0002-3407-5927ТрубниковаН. Н.TrubnikovaN. N.

Трубникова Надежда Николаевна — зав. лабораторией фундаментальных исследований

Донецк

Nadezhda N. Trubnikova — Head of the Laboratory of Fundamental Research

Donetsk 

orenaji3@bk.ruhttps://orcid.org/0009-0006-6404-2930КисиленкоИ. А.KisilenkoI. A.

Кисиленко Ирина Александровна — младший научный сотрудник

Донецк

Irina A. Kisilenko — Junior Researcher 

Donetsk 

irinka.dn.15@gmail.comhttps://orcid.org/0000-0001-7053-4428СолоповМ. В.SolopovM. V.

Солопов Максим Витальевич — научный сотрудник 

Донецк

Maxim V. Solopov — Researcher 

Donetsk 

mxsolopov@yandex.ruhttps://orcid.org/0000-0002-6461-4904ТурчинВ. В.TurchinV. V.

Турчин Виктор Васильевич — старший научный сотрудник

Донецк

Viktor V. Turchin — Senior Researcher 

Donetsk 

turchin.dn@mail.ruФГБУ «Институт неотложной и восстановительной хирургии имени В.К. Гусака»РоссияV.K. Gusak institute of emergency and reconstructive surgeryRussian FederationФГБНУ «Национальный научно-исследовательский институт общественного здоровья имени Н.А. Семашко»РоссияN.A. Semashko National Research Institute of Public HealthRussian FederationФГБОУ ВО «Ярославский государственный медицинский университет»РоссияYaroslavl State Medical UniversityRussian Federation202630032026416776Copyright © Филимонов Д.А., Котловский М.Ю., Потапов М.П., Белоцерковская М.А., Попандопуло А.Г., Трубникова Н.Н., Кисиленко И.А., Солопов М.В., Турчин В.В., 20262026Филимонов Д.А., Котловский М.Ю., Потапов М.П., Белоцерковская М.А., Попандопуло А.Г., Трубникова Н.Н., Кисиленко И.А., Солопов М.В., Турчин В.В.Filimonov D.A., Kotlovskiy M.Y., Potapov M.P., Belotserkovskaya M.A., Popandopulo A.G., Trubnikova N.N., Kisilenko I.A., Solopov M.V., Turchin V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pomph.ru/jour/article/view/224Актуальность

Актуальность. Рецептор 1, ассоциированный со следовыми аминами-1 (TAAR1) организует передачу сигнала через широкий спектр агонистов, которые функционируют как нейромодуляторы в классических нейромедиаторных системах, а также потенциально могут оказывать нейропротекторное действие.

Целью данной работы было определение паттерна экспрессии TAAR1 в клетках и тканях головного мозга с помощью анализа общедоступных баз данных экспрессии генов.

Материалы и методы

Материалы и методы. Для анализа экспрессии гена TAAR1 использовались наборы данных Института науки о мозге Аллена (Human Brain Atlas Microarray, Allen Brain Cell Atlas, Brain Allen Developing Human Brain Atlas, Whole Mouse Brain Transcriptomic Cell Type Atlas).

Результаты

Результаты. Анализ открытых транскриптомных баз данных показал экспрессию TAAR1 в коре больших полушарий, миндалине, базальных ганглиях, гиппокампе и таламусе человека. Выраженные различия между данными человека и мыши указывают на вероятные межвидовые особенности экспрессии TAAR1.

Заключение

Заключение. TAAR1 демонстрирует гетерогенное и преимущественно низкоуровневое распределение в структурах центральной нервной системы, что подчёркивает необходимость применения современных методов одноядерного/одноклеточного секвенирования и развития in vivo подходов к визуализации рецепторов.

Relevance

Relevance. Trace amine-associated receptor-1 (TAAR1) mediates signaling through a broad spectrum of agonists that function as neuromodulators in classical neurotransmitter systems and may also potentially exert a neuroprotective effect.

Objective

Objective. The aim of this study was to determine the expression pattern of TAAR1 in brain cells and tissues by analyzing publicly available gene expression databases.

Materials and methods

Materials and methods. TAAR1 gene expression was analyzed using datasets from the Allen Institute for Brain Science (Human Brain Atlas Microarray, Allen Brain Cell Atlas, Brain Allen Developing Human Brain Atlas, and Whole Mouse Brain Transcriptomic Cell Type Atlas).

Results

Results. Analysis of public transcriptomic databases revealed TAAR1 expression in the human cerebral cortex, amygdala, basal ganglia, hippocampus, and thalamus. Marked differences between human and mouse data indicate probable interspecies differences in TAAR1 expression.

Conclusion

Conclusion. TAAR1 exhibits a heterogeneous and predominantly low-grade distribution within the central nervous system, highlighting the need for modern single-nucleus/single-cell sequencing methods and the development of in vivo approaches to receptor imaging.

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The authors declare that there are no conflicts of interest present.